Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Endogenous glucocorticoids are required for normal macrophage activation and gastric Helicobacter pylori immunity
doi: 10.1152/ajpgi.00114.2024
Figure Lengend Snippet: Disruption of glucocorticoid signaling in the myeloid compartment protects from Helicobacter felis -induced gastric atrophy. A : representative hematoxylin-eosin (H&E) micrographs of the gastric corpus lesser curvature. KO, knockout; WT, wild type. Scale bars, 100 µm. B : representative immunostaining. Sections were probed with antibodies against the MIST1 (mature chief cells, green), the H + -K + -ATPase (parietal cells, red), and the Griffonia simplicifolia lectin (GSII; mucous neck cells, gray). Nuclei were stained with DAPI. Scale bars, 100 µm. C : quantitation of the number of parietal cells and chief cells ( n ≥ 5 mice/group). HF, H. felis ; Veh, vehicle. Data are means ± SD. * P ≤ 0.05, ** P ≤ 0.01, **** P ≤ 0.0001.
Article Snippet: Fluorescent conjugated Griffonia simplicifolia lectin (GSII; ThermoFisher Scientific) was added with secondary antibodies.
Techniques: Disruption, Knock-Out, Immunostaining, Staining, Quantitation Assay